Insertion and glycosylation of Pf3-derived membrane proteins in microsomes
نویسندگان
چکیده
منابع مشابه
Hydrophobic forces drive spontaneous membrane insertion of the bacteriophage Pf3 coat protein without topological control.
Bacterial integral inner membrane proteins are either translocated across the lipid bilayer using an energy-driven enzyme, such as the Sec translocase, or they might interact directly with the membrane due to hydrophobic forces. We report that the single-spanning Pf3 coat protein is spontaneously inserted into the membrane of Escherichia coli and requires the electrical component of the membran...
متن کاملiMembrane: homology-based membrane-insertion of proteins
iMembrane is a homology-based method, which predicts a membrane protein's position within a lipid bilayer. It projects the results of coarse-grained molecular dynamics simulations onto any membrane protein structure or sequence provided by the user. iMembrane is simple to use and is currently the only computational method allowing the rapid prediction of a membrane protein's lipid bilayer inser...
متن کاملIn vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.
We have characterized the early biosynthetic forms of the Torpedo electroplax acetylcholine receptor by using a cell-free protein synthesizing system. We obtained primary translation products of approximately 38, 50, 49, and 60 kilodaltons for the alpha, beta, gamma, and delta polypeptides, respectively, by using immunoprecipitation with subunit-specific antisera. These chains could each be lab...
متن کامل0 - Glycosylation of Proteins by Membrane Fractions of
In order to investigate 0-glycosylation of proteins in the fungus Trichoderma reesei QM 9414, a membrane preparation was isolated and used to study the glycosylation of endogenous proteins. Exogenously added GDP-[ U-4C]mannose was used to mannosylate both endogenous lipid and protein. The kinetics of mannosylation together with pulse-chase experiments with cold GDP-mannose revealed that lipid w...
متن کاملMembrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.
Membrane assembly was observed to proceed in cell-free extracts. Specifically, the membrane glycoprotein of vesicular stomatitis virus was synthesized in crude extracts of wheat germ in the presence of membrane vesicles derived from pancreatic endoplasmic reticulum. The resulting glycoprotein spans the lipid bilayer asymmetrically, is glycosylated, and is indistinguishable in these respects fro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 2002
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(02)02312-8